Assignment Bioassay Of Androgenic Hormones - 1412 Words

Faculty of Pharmacy Pharmacology Department Assignment Topic: Bioassay of androgenic hormones Course Title: Bioassay Course Code: PO411 Lab Group: A1 Submitted to: Dr Mona Prepared by: Rahma Ahmed Ahmed - 141191 Due date: 10-27-2016 - Fall 2016 - Androgen Bioassays Introduction: During the recent decades, athletes were trying to enhance their performance by using exogenous hormones such as; androgens, erythropoietin and growth hormone. The most potent form of sports steroids is the Androgens and it is the most common type of steroids that enhances the performance that can be detected in screening tests. Detection of androgens is through urine screening to detect even trace amounts of androgens. Gas chromatography-mass spectrometry (GC-MS) is a highly specific and sensitive method for detection of steroids in the screening urine samples. In a trial to pass the screening methods, designer androgens was created with different chemical structures to known androgens so it cannot be easily detected by GC-MS. Designer androgens can be detected by androgen bioassays because they can activate the androgen receptor (AR). Therefore, androgen bioassays maybe a suitable tool for screening of biological samples suspected to contain an androgen. . Receptor binding assays: Principle: The ligand competes for binding to AR. Receptor binding assays have been developed to measure compounds, including hormones, that are able to bind specifically to receptors. Procedure: the